ABSTRACT
OBJECTIVE:To establish the method for content determination of cantharidin in Lytta caraganae,and to use the result as the extract screening evidence of L. caraganae source material. METHODS:Ultrasonic extraction method was used to extract cantharidin from L. caraganae using acetone as solvent. HPLC method was adopted to determine the content of cantharidin. The determination was performed on C18column with mobile phase consisted of methanol-water(23:77)at flow rate of 1.0 mL/min. The detection wavelength was set 230 nm,the column temperature was set at 35 ℃,and sample size was 10 μL. The content of cantharidin in L. caraganae was determined and compared with the results of content determination by the method stated in Chinese Pharmacopeia(using chloroform as extraction solvent). RESULTS:The liner range of cantharidin were 0.2-1.0 mg/mL (r=0.998 8)with average methodology recovery rates of 101.1%(RSD=1.7%,n=6). The average content of cantharidin in L. caraganae was 0.932%(n=3),while the content of cantharidin was 0.793%(n=3)determined by the method stated in Chinese Pharmacopeia. Both were higher than the requirement of Chinese Pharmacopeia that the content of cantharidin in scource material of cantharidin was higher than 0.35%. CONCLUSIONS:Established method is accurate and reliable for the content determination cantharidin in L. caraganae. The content of cantharidin is up to the standard of Chinese Pharmacopeia,and can be used as source material for exacting cantharidin.
ABSTRACT
OBJECTIVE: To prepare heme from cherry valley duck blood. METHODS: Single factor experiments and orthogonal experiment were carried out to establish the optimal process of preparing heme from cherry valley duck blood by a new microwave-assisted acid acetone extraction method. RESULTS: Single factor experiments proved that the microwave-assisted extraction process significantly improved the extraction efficiency of heme. In addition, orthogonal experiment indicated the power of microwave and microwave extraction time had significant effects on the extraction results, and the optimum conditions of microwave-assisted extraction in laboratory were as follows: the power was 800 W, extraction time was 60 min, and the temperature was 50°C. CONCLUSION: The new microwave-assisted method, combining cell disruption and heme extraction process, can simplify the traditional acid acetone extraction process, and it is proved to be feasible.